Applications of synchrotron infrared microspectrometry in biological

tissue and forensic science


Yao-Chang Lee* and Ching-Iue Chen

National Synchrotron Radiation Research Center, Hsinchu, Taiwan

No. 101 Hsin-Ann Road, Hsinchu Science Park, Hsinchu 30077, Taiwan


The advantage of the synchrotron source is high throughput at high spatial resolution. And the infrared output of the synchrotron beam line was fed into a IR microscope as an alternate infrared light source. The coupling of infrared microscope and synchrotron source produces the highest signal-to-noise ratio (SNR) spectrum with the highest spectral resolution from the smallest sample area. Infrared spectroscopic imaging uses a single element detector associated with an imaging spectrometer to produce an array of spectra over a sample.

Fourier transform infrared (FT-IR) microspectroscopy was used to image and subsequently produces spectral images of the distribution chemical components in biological tissue and forensic sample. The wing of moth (Amata perixanthia), human hair section, compact disc, and resolution target (USAF target) were examined in 10 um × 10 um of spatial resolution. The end result is infrared spectral images of the sample, enabling constituents and composition to be visualized easily. This technique can be applied to samples of a wide range of sizes and areas of interest. The vibration frequencies of chemical component utilized to produce chemical images were based on their characteristic frequencies for indicating each component to the exclusion of others in the matrix, by comparison of the spectra of pure components. This information is available to a certain extent in normal infrared microscopy and enhanced in synchrotron powered infrared microscopy.




Characteristics of silicon nanowire devices in biological molecules detection

J. T. Sheu1, 3*, K. S. You2, C. C. Chen1, S. C. Lin3 and K. S. Liang3

Department of Electric Engineering, National Chi Nan University, No. 1 University Rd. Puli 545, Taiwan, 2National Chiao Tung University and 3National Synchroton Radiation Research Center

Mailing address:No. 1 University Rd., Puli, Nantou 545, Taiwan


Silicon nanowire (SiNW) biological detection devices were fabricated by scanning probe lithography (SPL) and tetra-methyl-ammonium hydroxide (TMAH) wet etching process on (110)-oriented n-type SOI wafer. The device structure was imaged by atomic force microscope (AFM). The silicon nanowire with 40 nm in height and 2 µm in length were connected to the electrodes of source and drain. And the line width of silicon nanowire was 60 nm and observed from the image of scanning electron microscope. The surface of SiNW was then modified by amino-propyl-trimethoxy-silane (APTS) and bis-sulfo-succinimidyl suberate (BS3) to activate the surface of the silicon dioxide layer around the SiNW to obtain a protein-bindable environment. APTS modified the silicon dioxide layer by reacting with silanol groups to generate an amino-derivatized surface, which changed the surface potential of SiNW from negative to positive and resulted in change of conductance underneath such that the turn-on voltage (Id = 10 nA) of SiNW device moved from 3.64 V to 0.72 V. This surface modification of APTS molecules changed the work function in the interface and performed like a positive bias on the bottom. The modifications of the SiNW-SiO2-APTS surface with linking BS3 and IgG molecules also observed the changes on turn-on voltages. Bioconjucation of biomolecules on the surface of SiNWs is achieved by providing solution through microfluidic channel. Sensitivity of SiNW devices as biomolecular detection and selectivity of different biomolecules will be discussed. The schematic of device structure is shown in figure1.





Transmission X-ray Microscopy at NSRRC

Mau-Tsu Tang*, Cheng-Hao Ko, Yen-Fang Song, Te-Hui Lee, Gung-Chian Yin,

Ying-Huang Lai, King-Long Tsang and Keng S. Liang

National Synchrotron Radiation Research Center, 101 Hsin-Ann Road,

Hsinchu Science Park, Hsinchu 30077, Taiwan


The NSRRC is developing its hard x-ray imaging capacity for nano-scale structural probing. The designed transmission x-ray microscope operating in 8~11keV photon energy range is under construction and will be installed into the beamline in this September. Zone plate optics with Zernike’s phase contrast mechanism is incorporated into the microscope to provide 3D tomographic “virtual slicing” imagings with 60nm spatial resolution in a 15μm×15μm field of view. Examples of potential applications include the analysis of failure mechanisms in microelectronic devices, the characteristics of porous materials such as soils and rock in geo- and environmental sciences. In life science, the “virtual slicing” of the 3D imaging, while in conjugated with proper labeling agents, will potentially be able to view specific cellular function in-situ either in a single cell or any region of a tissue.




Optical Biopsy of Normal, Fibrotic, and Tumor Liver Tissues Using Multiphoton Microscopy


Liu Yuan1*, Peter T. Fwu1*, Hsuan-Shu Lee2, and Chen-Yuan Dong1

1Department of Physics, National Taiwan University, 106, Taipei, Taiwan and

2Department of internal medicine, National Taiwan University Hospital, 100, Taipei, Taiwan

No.1, Sec. 4, Roosevelt Road Section 4, Taipei, 106, Taiwan


Liver fibrosis and tumors are serious diseases and their effective detection in vivo can lead to early diagnosis and the development of effective treatment procedures. However, conventional histological procedures limit the ability to diagnose liver tissues in living specimens and the development of intravital imaging techniques is invaluable in following liver physiology in vivo. A possible solution is to apply multiphoton microscopy, a powerful and minimally invasive, three-dimensional imaging modality, to the diagnosis of liver tissues. In this work, we test the feasibility of this approach by performing ex vivo, multiphoton imaging of liver tissues in different states. Normal, fibrotic, and tumor specimens of the liver are examined and we will present the results showing the differences between the three tissue types.




Monitoring the Thermally Induced Collagen Structural Transitions

Using Second Harmonic Generation Microscopy

S.W. Teng1, C. Y. Hsiao2, S.J. Lin3, H.Y. Tan4, C.Y. Dong1

1Department of Physics, National Taiwan University, Taiwan

No.1, Sec.4, Roosevelt Road, Taipei, Taiwan 106

2Department of Electrical Engineering, National Taiwan University, Taiwan

No.1, Sec.4, Roosevelt Road, Taipei, Taiwan 106

3National Taiwan University Hospital, Taiwan

No.7. Chung San South Road, Taipei, Taiwan

4Chang – Gung Memorial Hospital, Taiwan

No.199. Tnughwa Rd. , Taipei, Taiwan


Our research focuses on the thermal disruption of type I collagen(bovine tendon and porcine cornea) is investigated using second harmonic generation (SHG) microscopy. We investigated the effects of second harmonic generation images and intensity as the temperature increasing . We found a structural transition at about 60~65where a drop in second harmonic intensity is correlated to a macroscopic disorganization of the collagen fibers. Our results suggest that SHG microscopy is an effective technique to monitor thermally induced collagen structural changes in biomedical applications.