第八屆生物物理新知研討會

The 8th Symposium on Recent Advances in Biophysics

 

Applications of synchrotron crystallography: Some recent examples.

 

Andrew H.-J. Wang, Department of Biochemistry, University of Illinois at Urbana-Champaign, Urbana, IL 61801 and Institute of Biological Chemistry, Academia Sinica, Nankang, Taipei, 11529, Taiwan, ROC

 

Recent advances in structural biology have been remarkable. Many interesting proteins that have not been available previously for any biochemical and biophysical studies can now be overproduced by genetic cloning techniques and subjected to structural analysis. Often those biological systems are very complex and large in size and their crystals may be very small and have limited lifetimes. Synchrotron radiation is the only way to solve the crystal structure of those molecules. Moreover, the new structures are determined at much higher resolution (better than 1 Å), providing very accurate structural information which is important in understanding their functions (e.g., enzyme active site).

Our laboratory is interested in the 3D structure and the molecular interactions of biological macromolecules, in particular nucleic acids and proteins. Here I describe some recent examples of new structures solved by synchrotron crystallography.