AFFINITY CAPILLARY ELECTROPHORESIS: FROM BINDING MEASUREMENT TO LIBRARY OPTIMIZATION AND SCREENING
YEN-HO CHU, Department of Chemistry, National Chung Cheng University, Chia-Yi, Taiwan 621
Affinity capillary electrophoresis is a useful procedure for the studies of biomolecular recognitions. Since its introduction in early 1990s, this method has proved valuable in chiral separation of racemates, the measurement of binding constants, the estimation of kinetic rate constants, the determination of stoichiometries of binding interactions, and, most recently, the optimization and screening of combinatorial libraries in solutions. This technique uses small amounts of samples, involves no radiolabeled materials and chemically immobilized ligands, and does not require changes in spectroscopic characteristics upon binding. This presentation will describe our recent progress in affinity capillary electrophoresis.